Genetic association analysis of rs662799 ( − 1131A > G) polymorphism of APOA5 gene with morphometric and physio-metric traits using multiplex PCR

Abstract Background The apolipoprotein A5 ( APOA5 ) gene, significantly expressed in liver, has been involved regulation of triglyceride metabolism, plasma lipid levels, serum adipokine levels and cardiovascular traits. A single-nucleotide polymorphism rs662799 − 1131A > G), 2 Kb upstream the promoter region this causes decrease concentration product coded by gene; hence, it may be responsible for impairments normal function ultimately leading to disease condition. Keeping view importance aim present study was examine association genetic variant gene with two quantitative traits simultaneously, viz. body mass index blood pressure. Results a population 246 subjects from North Indian region. Measurements morphometric physio-metric parameters were recorded using standard measures. Genotyping (rs662799) Tetra-primer amplification refractory mutation system PCR performed. Statistical analyses carried out MS-Excel SigmaPlot, significance level setup as p < 0.05. allelic distribution 77% major allele (A) 23% minor (G). Significant increased pressure observed, presence G. Under recessive model, conferred 17.71-fold risk elevated (OR = 17.71, 0.001, CI (95%) 4.05–77.46), increase pressure, 3.79- 3.83-fold systolic diastolic 3.792, 0.023, 1.25–11.509 OR 3.83, 0.012, 1.375–10.68, respectively) observed. dominant showed 3.060-fold 3.060, 1.78–5.25). Conclusions G significant susceptible BMI BP. This helpful clinicians researchers investigate diagnostic prognostic value question.